Bioinsight offers contract RNA services for researchers working on gene function — from expression analysis through to the synthesis of the RNA molecules used to test it. We support both model and non-model organisms.
RNA-seq analysis
RNA-seq is used to compare gene expression comprehensively between treatment and control, between tissues, between developmental stages, or across environmental conditions. We support the work from raw data through to a shortlist of candidate genes: extraction of differentially expressed genes, functional annotation, pathway analysis and organisation of candidates. Downstream work such as qPCR validation and RNAi experiments can also be arranged.
Who this is for
- You want to find genes whose expression changes between treatment and control
- You already have RNA-seq data but are unsure how to analyse or interpret it
- You are searching for candidate genes in a non-model organism
- You want to narrow a list of differentially expressed genes down to the functionally important ones
- You need figures and tables suitable for a paper or report
Available analyses
| Step | Description |
|---|---|
| FASTQ quality check | Data quality, read counts and base quality |
| Trimming | Removal of low-quality sequence and adapters |
| Mapping | Alignment to a reference genome or reference sequence |
| De novo assembly | Transcript reconstruction for organisms without a reference genome |
| Expression estimation | Quantification per gene and per transcript |
| Differential expression | Extraction of genes that change between conditions |
| Functional annotation | Homology search and inference using public databases |
| Visualisation | PCA, heatmaps, volcano plots and similar |
| Candidate gene extraction | Gene lists built around the aim of the study |
| Reporting | Methods, results and discussion, organised |
Custom dsRNA synthesis
Double-stranded RNA is widely used as a tool for suppressing the expression of a specific gene. We synthesise research-use dsRNA from sequences you specify, for gene knockdown experiments, functional analysis of target genes and comparative evaluation of candidate sequences.
| Item | Specification |
|---|---|
| Service | Research-use dsRNA synthesis from customer-specified sequences, by in vitro transcription |
| Length | Approximately 300–1,000 bp (other lengths on request) |
| Scale | Tens of micrograms to hundreds of milligrams, depending on application |
| Organisms | Insects, plants, microorganisms, cultured cells and other research targets |
| Quality control | Size confirmation by agarose gel electrophoresis, concentration measurement |
| Deliverables | dsRNA solution, QC results (gel image, concentration), lot information |
| Lead time | Approximately 2–4 weeks, depending on sequence and quantity |
Custom siRNA synthesis
Research-use siRNA synthesised from sequences you specify, for RNA interference knockdown experiments, functional analysis of target genes and comparison of candidate sequences.
| Item | Specification |
|---|---|
| Service | Research-use siRNA synthesis from customer-specified sequences |
| Structure | Double-stranded siRNA, sense and antisense strands supplied annealed |
| Organisms | Cultured cells, insects, plants and other research targets |
| Quality control | Mass spectrometry, concentration measurement |
| Delivery form | siRNA solution or lyophilised, with QC results |
| Quantity | Discussed individually according to requirement and application |
How to order
- Enquiry — we confirm the target organism, target gene, intended use and quantity required.
- Sequence review and quotation — we propose a synthesis approach and quotation based on the sequence information you provide.
- Synthesis — carried out to the specified sequence.
- Quality control — size confirmation and concentration measurement.
- Delivery — product and QC results.
Please store dsRNA and siRNA at −20 °C or below. Repeated freeze-thaw cycles degrade quality, so we recommend aliquoting before use.
Sequences we cannot synthesise
We are unable to accept orders for sequences related to the virulence or replication of pathogenic viruses and microorganisms, sequences that could confer or enhance infectivity, and sequences associated with select agents or toxins.
Enquiries
Please tell us the target organism, target gene, intended use and quantity, and we can give you an indicative quotation and schedule in our first reply. We normally respond within two business days.